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KMID : 0665220170300010051
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Korean Journal of Food and Nutrition
2017 Volume.30 No. 1 p.51 ~ p.58
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Effect of Extraction Solvent on the Antioxidant Activity of Lentinula edodes GNA01 Extract
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Jang Hye-Lim
Park Seo-Yeon
Nam Jin-Sik
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Abstract
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Antioxidant activities of 80% methanol, water, and 70% ethanol extracts of Lentinula edodes GNA01 were compared and estimated. The yield of Lentinula edodes GNA01 was identified to be in the following order: water>70% ethanol>80% methanol, but there was no significant difference between 80% methanol and 70% ethanol extracts. The highest total phenolic content (TPC) and total flavonoid content (TFC) were found in water extract, and TPC of 80% methanol extract was higher than that of 70% ethanol extract and TFC of 70% ethanol extract was higher than that of 80% methanol extract. Water extract exhibited the strongest DPPH, ABTS radicals, and nitrite scavenging activities, Fe2+ chelating ability, and FRAP among the three extracts. In addition, antioxidant activity of 80% methanol extract was higher than that of 70% ethanol extract in most of the experiments. As a result, antioxidant activity of Lentinula edodes GNA01 showed a difference according to extraction solvent and concentration; nevertheless, water extract exhibiting high polarity had the strongest antioxidant effect. Consequently, water extract from Lentinula edodes GNA01 is anticipated to be useful for the development of a high value-added functional product.
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KEYWORD
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Lentinula edodes GNA01, antioxidant activity, DPPH, ABTS, Fe2+ chelating
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